0304382 Molecular Biology (Credit Hours:3)
Course Description :Prerequisite: 0304341 The lectures in this course covers the following topics; historical back ground; chemistry of nucleic acid; Watson-Crick model of DNA; physical and chemical properties of nucleic acids; an introduction to gene function (selection, transcription and translation); transcription in prokaryotic cells; regulation of transcription in prokaryotic cells; transcription in eukaryotic cells; regulation of transcription in eukaryotic cells; general and specific transcription factors; post transcriptional events ; translation and the genetic code; post translational events; DNA replication in prokaryotic and eukaryotic cells; Mutation and DNA repair. the laboratory covers the following topics: Isolation of nucleic acids; quantitative and qualitative measurements of nucleic acids; the use of restriction enzymes; Amplification of nucleic acids; characterization and manipulation of the recombinant plasmid pGLO containing GFP gene; bacterial transformation and gene expression and protein produced isolation ; DNA-cloning and southern blot.
Department :Biological Sciences
Program :Bachelor of Biological Sciences
Course Level :Bachelor
Course Outline :
0334382-Molecular Biology -CS-2 _2018.pdf